Expression cloning of the epithelial sodium channel
نویسندگان
چکیده
منابع مشابه
Cloning and functional expression of the mouse epithelial sodium channel.
The epithelial sodium channel (ENaC) plays a major role in the transepithelial reabsorption of sodium in the renal cortical collecting duct, distal colon, and lung. ENaCs are formed by three structurally related subunits, termed α-, β-, and γENaC. We previously isolated and sequenced cDNAs encoding a portion of mouse α-, β-, and γENaC (α-, β-, and γmENaC). These cDNAs were used to screen an oli...
متن کاملNedd4-2 isoforms ubiquitinate individual epithelial sodium channel subunits and reduce surface expression and function of the epithelial sodium channel.
We previously reported the existence of multiple isoforms of human Nedd4-2 (Am J Physiol Renal Physiol 285: F916-F929, 2003). When overexpressed in M-1 collecting duct epithelia, full-length Nedd4-2 (Nedd4-2), Nedd4-2 lacking the NH(2)-terminal C2 domain (Nedd4-2DeltaC2), and Nedd4-2 lacking WW domains 2 and 3 (Nedd4-2DeltaWW2,3) variably reduce benzamil-sensitive Na(+) transport. We investigat...
متن کاملRenal sodium handling: the role of the epithelial sodium channel.
T he epithelial sodium channel (ENaC) is a critically important final regulator of the balance between intake and excretion of dietary sodium (1), and along with the thiazide-sensitive NaCl co-transporter constitutes the predominant sodium transport systems in the aldosterone-sensitive distal nephron (2). The use of the oocyte expression system for cloning the ENaC subunits was a technical tour...
متن کاملFunctional domains of the epithelial sodium channel.
T he epithelial sodium channel (ENaC) plays an important role in ion transport of many organ systems. In kidney, colon, and sweat gland, ENaC-mediated transepithelial sodium transport helps to adjust sodium excretion in the urine, feces, and sweat to fulfill homeostatic requirements. In lung and airways, ENaC activity is important for alveolar liquid clearance and regulation of mucous fluidity....
متن کاملExtracellular chloride regulates the epithelial sodium channel.
The extracellular domain of the epithelial sodium channel ENaC is exposed to a wide range of Cl(-) concentrations in the kidney and in other epithelia. We tested whether Cl(-) alters ENaC activity. In Xenopus oocytes expressing human ENaC, replacement of Cl(-) with SO4(2-), H2PO4(-), or SCN(-) produced a large increase in ENaC current, indicating that extracellular Cl(-) inhibits ENaC. Extracel...
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ژورنال
عنوان ژورنال: Kidney International
سال: 1995
ISSN: 0085-2538
DOI: 10.1038/ki.1995.376